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Description
Human GR-b ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Glucocorticoid Receptor Beta (GR-b) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Glucocorticoid Receptor Beta (GR-b) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Glucocorticoid Receptor Beta ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Glucocorticoid receptor β (GR-β) is one subtype of the glucocorticoid receptor, the other being GRα. Both are derived from the same transcript of the glucocorticoid receptor gene, spliced differently. GRα can bind to DNA but not to steroids, potentially acting as a steroid inhibitor by interfering with GRα's DNA binding. GRα and GRβ are identical in their first 727 amino acids. From amino acid 728 onward, GRα has a 50-amino acid sequence, while GRβ has only 15. At the mRNA level, both contain exons 1 through 8, differing in that GRα contains exons 9α and GRβ contains exons 9β. GRα is expressed in virtually all tissues and cells, and its level far exceeds that of GRβ in most cells. Glucocorticoids primarily exert their biological effects by binding to GRα. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.7 ★★★★★
Based on 12 reviews
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Product Reviews
★★★★★ 5
All Futurama fans should have it!
Format: Hardcover
Amazing book!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 18, 2026
★★★★★ 5
A must buy for animators/animation students
Format: Hardcover
This book spares no detail. I've had my fair share of art books before and this one is a gem, it gives you a lot for a low price, get it!!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 15, 2025
★★★★★ 4
Good news, everyone! The Art of Futurama is here!
Format: Hardcover, Format: Hardcover
I always thought Futurama was simple and fun! However, after reading this book, I now understand that beneath its apparent simplicity lies an entire universe of complex experience, research, innovation, hard work, and talent.
★★★★☆ Art
Unlike many other artbooks that rely heavily on production stills, this one is 90% sketchbook and finalized line art designs—a rare and delightful find. You'll not only discover the initial designs for all the main characters but also learn how to draw them. Each curve and line in the characters' designs is intentional, contributing to their unique personalities.
The book also features three fold-out sections: New New York's buildings and junkyard, the Nimbus ship in orbit, and the Planet Express. While these offer detailed designs for enthusiasts, I didn’t find them particularly necessary, as there’s little in them that demands close study.
★★★★☆ Text
The story of Futurama's creation is a joy to read. The book is divided into a few intros and nine chapters, each featuring two pages of text that include quotes from interviews with the show’s creators about their memories of its production. Additionally, there’s a wealth of quotes accompanying the images.
I especially enjoyed learning how the team found inspiration for New New York by purchasing reference books in New York City bookstores. The integration of 3D and 2D animation—which was revolutionary at the time—is also well-documented.
However, the advertised Futurama episode guide feels redundant. It offers nothing beyond episode titles and omits seasons 8 and 9 entirely, with no explanation given for their exclusion.
★★★★☆ Book Design
The book's design features a creative transparent front cover case, a landscape layout, well-placed text and images, and a large, easy-to-read font. The page numbers are conveniently placed at the center of the page edge, making navigation effortless.
Despite its strengths, I still feel the fold-outs are unnecessary and could have been omitted without any loss of value.
★★★★★ Price
This book offers excellent quality for its price! Released in November 2024, it’s available as of January 2025 for $25. To paraphrase one of Futurama's opening chyron jokes (the texts below the show's logo at the beginning of each episode): "If you don’t buy it, someone else will."
★★★★☆ Overall Impressions
It took me two relaxed sessions to fully read this book, and I can confidently say it’s impossible to get bored with it (or is that just nostalgia talking?). The hundreds of well-crafted line art illustrations of characters, assets, and backgrounds showcase the creators’ immense talent.
The accompanying text is both engaging and informative, and the early sketches of the main characters provide a fascinating narrative of their development.
That said, I was left wanting more. At 176 pages, the book feels a bit short, especially since it doesn’t include a single chapter dedicated to seasons 8 and 9, which aired after a ten-year hiatus.
Nevertheless, The Art of Futurama is an excellent addition to any home library or a thoughtful gift for fans who grew up watching this iconic show in the late 1990s and 2000s.
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Reviewed in the United States on January 5, 2025
★★★★★ 5
The book every Futurama fan should have
Format: Hardcover
Wonderful book on the behind the scenes making of Futurama. Lots of great concept art with commentary on the making of this outstanding series. If you love Futurama then you need this book. Highly recommended!!!! A++++++
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Reviewed in the United States on July 19, 2025
★★★★★ 5
Great for Futurama fans!
Format: Hardcover
Really good quality of prints and pictures. Bought it on sale just for 13$, totally worth the price!!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 27, 2025
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