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Description
Human CASP7 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Caspase 7 (CASP7) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Caspase 7 (CASP7) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Caspase 7 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Caspase 7 (CASP7) is a protein encoded by the CASP7 gene. CASP7 homologs have been found in nearly all mammalian species for which complete genomic data are available. It is a member of the caspase (cysteine aspartate protease) family and has been shown to be the executioner of apoptosis. Sequentially activated caspases play a central role in the execution phase of apoptosis. Caspases exist as inactive proenzymes. Proteolytic processing by upstream caspases (caspase-8 and -9) at conserved aspartate residues produces two subunits, a large and a small, which dimerize to form the active enzyme as a heterotetramer. This procaspase is cleaved by caspase 3, caspase 10, and caspase 9. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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★★★★★ 5
THE BEST. Seriously.
Size: 36E, Color: White
The world is upside down. How can a twenty dollar bra outperform my hundred dollar bras??? I don't know but here we are. I am 49, a 34F/36e, 5'1". Finding a great bra is challenging to say the least. This bra is it, y'all. I bought it in a different color a couple of weeks ago. I wore it three times in a row to make sure it wouldn't bag out, lose its support. I washed it, not in a lingerie bag, on warm, dried it on high, to make sure it would hold up to that. It passed both tests with flying colors. It is so comfortable. It feels wireless! It is very flattering, has great under-arm-flub-smoothing and support. The back is substantial and smoothing but without being bulky or restrictive. The straps are wide, but not too wide, and totally stay put all day. I bought the other three colors and the sizing is seamless across colors. This is a winner, folks!!!
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Reviewed in the United States on November 26, 2025
★★★★★ 5
Comfort & Support
Size: 40DD, Color: Black
OMG.... FINALLY..... I have ordered at least 40 bras in the last 3 years trying to find a bra that holds my chichis in, lifts, supports, separates n COMFORTABLE!!! Ladies... this is the one... im ordering more... price is right, so soft.... cups are soft... doesn't add size cuz i don't need it, not sheer so the titis are poking thru my tops.... I cant say enough about this bra.... absolutely love this bra and so relieved to finally find it. .. you won't be disappointed.... get it
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Reviewed in the United States on May 1, 2026
★★★★★ 4
Surprisingly Comfortable
Size: 36D, Color: Beige
I'm very surprised by the comfort of this bra. I've worn wireless padded/lined bras for as long as I can remember so this is quite a change from my norm. I am only 5' tall so having larger breasts tend to make me look top heavy. The minimizing effect makes my outfits look so much better and slims me down a bit. I am unsure of the sizing though. I purchased my normal size in a 36D but the top of the cups have some wrinkling as if I'm not quite filling the cup. It does smooth out a bit after I've been wearing it for a while. The lift is nice but not extreme. It more puts the girls where they were about 20 years ago but it doesn't do much for shaping. Overall it's a nice bra and I'd likely purchase again if I could just get the sizing right.
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Reviewed in the United States on March 1, 2026
★★★★★ 3
DELIMIRA Women's Inbarely Lift Full Coverage Bra
Size: 48D, Color: Black
I have had this bra now for 4 months. It is VERY comfortable. However, after only 4 months of use, the cup wire is sticking out of the outer sides. It hurts when it pokes me and digs into my skin. If the wire wouldn't have stuck out, it would have been PERFECT. I LOVED this bra. It's a real shame!
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Reviewed in the United States on April 8, 2026
★★★★★ 5
They should call it The Unicorn Bra
Size: 42F, Color: White
What kind of sorcery is this?! I am 58 and have had huge boobs my entire life. I've spent thousands of dollars on bras over the years and have never been 100% happy with one of them. Until now. My daughter recently lost a ton of weight and was looking for "in between" bras until she hits her goal. She didn't want to spend a lot each time she had to replace one so she researched and found Delmira. She immediately called to tell me she found a Unicorn and of course, I had to try it. I ordered 4 more in different colors and styles and let me tell you...all of them are amazing. The support, comfort, style...simply amazing!!
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Reviewed in the United States on February 22, 2026