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Description
Human neurokinin-1 receptors,nk-1r ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against neurokinin-1 receptor (NK-1R). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the presence of neurokinin-1 receptor (NK-1R) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Neurokinin-1 receptor ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Neurokinin-1 receptor (NK-1R), also known as tachykinin receptor 1 (TACR1) or substance P receptor (SPR), is a G protein-coupled receptor encoded by the TACR1 gene and found in the central and peripheral nervous systems. Its endogenous ligand is substance P, although it has some affinity for other tachykinins. It consists of 407 amino acid residues with a molecular weight of 58,000. It, along with other tachykinin receptors, consists of seven hydrophobic transmembrane (TM) domains with three extracellular and three intracellular loops, an amino terminus, and a cytoplasmic carboxyl terminus. It can be found in both the central and peripheral nervous systems. It is present in neurons, brainstem, vascular endothelial cells, muscle, gastrointestinal tract, genitourinary tract, lung tissue, thyroid gland, and various immune cells. Binding of substance P is involved in the transmission of stress signals and pain, smooth muscle contraction, and inflammation. Other diseases involving the NK-1 receptor system include asthma, rheumatoid arthritis, and gastrointestinal diseases. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.0 ★★★★★
Based on 5 reviews
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Product Reviews
★★★★★ 5
Works good
Works great, was easy to connect and it’s easy to use. It’s durable. Most of the time I’m using it instead of my fingers. Pairs well with my iPad A16. It’s not heavy, nor is it too light. Sometimes it’s annoying when I’m trying to use the keyboard on my iPad but it doesn’t pull it up since it’s connected to the pencil, but it’s not too big of a deal. I love the appearance, I’ve always been obsessed with this type of stylus, I found it more practical and easier to use than a traditional stylus. (Plus it makes it so much easier to exit out of ads) I won’t comment on the price since I’m sure it’s worth the money, but I’ll let others be the judge of that. For art purposes, I’m not an artist yet, but I’m practicing, and it works for me, I just can’t comment on how it’ll be for an actual artist though, so please do your research on which will be best for you!
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Reviewed in the United States on April 10, 2026
★★★★★ 4
Not perfect but a good pencil for a good price.
Not as user friendly as the original pencil and battery doesn’t seem to hold a charge very long. After about 4 hours of being charged it was down to 46%. That was intermittent use and non use, with about 3 hours actual use. To charge the original pencil I pulled off the end exposing the lightening connector and plugged it into the receptacle on my iPad. This requires a USB-C cable connection from either the charger or the iPad. Admittedly, it’s not that big a deal, but it feels like a step backwards from the original. The battery longevity is a little more irksome. The pencil is very easy to use, and I’m actually amazed at how the iPad is able to generate text when I write with it, even with my sometimes barely legible script. Much improved over the original. I can live with the minor irritations because it’s a good value for the price.
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Reviewed in the United States on November 20, 2025
★★★★★ 5
Sleek Design, Smooth Writing, and a Fun Tool for Artists and Everyday Use
The Apple Pencil (USB-C) is a streamlined and affordable option for users who want Apple’s signature precision without the premium price of higher-end models. Designed primarily for note-taking, sketching, and everyday creative tasks, it delivers the smooth, low-latency performance Apple Pencil is known for. Writing feels natural, with accurate pressure response and excellent palm rejection when used with supported iPads.
This version connects and charges via USB-C, making it convenient for users who prefer a simple wired setup and already rely on USB-C accessories. It’s lightweight, comfortable to hold, and magnetically attaches to compatible iPads for storage, though it does not support wireless charging or pairing. While it lacks advanced features like pressure sensitivity for artistic shading or double-tap gesture controls found in Apple Pencil (2nd generation), it still performs exceptionally well for students, professionals, and casual users.
However, a device compatibility check is essential before making a purchase. The Apple Pencil (USB-C) works only with select iPad models, and it does not support older Lightning-based iPads. Additionally, artists who rely on advanced drawing features may find this model somewhat limited.
Overall, the Apple Pencil (USB-C) is a well-built, reliable stylus that excels at core tasks. If your iPad is compatible and your needs are focused on writing, annotating, and basic drawing, it offers excellent value within Apple’s ecosystem.
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Reviewed in the United States on January 4, 2026
★★★★★ 5
Great laptop
Style: 256GB | Magic Keyboard, Color: Silver, Set: Without AppleCare+
The Neo has quickly become one of my favorite pieces of tech, and a big part of that comes down to two things Apple consistently gets right: MacOS and build quality.
This is my first MacOS product and it is a joy to use. Everything feels smooth, intuitive, and thoughtfully designed. From the seamless integration with other Apple devices to the clean interface and reliable performance, it just works in a way that lets me focus on what I’m doing instead of fighting with the system.
Then there’s the build quality, which is honestly second to none. The Neo feels incredibly solid the moment you pick it up. The aluminum chassis is sleek yet durable, with no flex or creaking, and the attention to detail is obvious in everything from the hinge to the keyboard. It has that premium feel that makes it clear you’re using a quality device. The trackpad is large and precise, easily the best I’ve used on any laptop, and the keyboard strikes a great balance between comfort and responsiveness.
Overall, the Neo delivers a polished experience that’s hard to beat. Between the reliability and elegance of MacOS and the exceptional build quality, it’s a laptop that feels worth the investment.
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Reviewed in the United States on April 9, 2026
★★★★★ 5
Impressive web PC, first time mac user, massive upgrade over a 4 year old Lenovo Yoga 13
Style: 256GB | Magic Keyboard, Color: Citrus, Set: Without AppleCare+
The neo is really impressive for $600. It has a nice screen, an incredibly rigid aluminum chassis, loads web pages and videos super fast, boots quick. Impressive Bluetooth and WiFi range and performance (Bluetooth is so much better then my old laptop), good speakers(for a laptop), good battery life, a decent keyboard, excellent trackpad, decent web cam, looks nice, low heat output and no vents to get blocked, compact and light. Highly recommended for anyone shopping for a compact web machine.
The only negatives on the hardware are: lack of keyboard backlight, the interior edge of the chassis is sharp, not enough to cut you, but enough to be annoying when typing in some positions, not many ports just a normal USB C and a 2.0 speed USB C (both can be used for charging) and screen does not fold flat, so the laptop cannot be stood up against the wall.
I was a bit unsure about the Neo because of it using a cellphone chip, but that was not a problem at all. The Neo is super snappy, as fast as my Workstation/Gaming PC when loading web pages and videos. Also I have not noticed any issues with the amount of RAM, 8GB seems sufficient for running a web browser, just as long as you do not go crazy on tabs in Chrome or if you use Firefox that actually manages tabs properly. As a web browser machine I doubt that this will have any performance issues. I am sure that you would hit limits of the mobile CPU if you do did something serious like video editing, but it is more then enough for more casual use.
As someone who had never used a mac before I was a bit apprehensive about trying a different OS, but really it has been fine. I mostly just use my laptop to run a web browser, so I do not interact with the OS that much and MacOS is really pretty nice in many ways. The hardest thing to get used to will be the copy paste shortcuts using command instead of ctrl. Mostly MacOS functions about the same as windows, just better. You can just shut the lid on the Neo and it actually shuts off without hardly using any battery and instantly resumes when you open it again. When you do shut it down boot time is super fast. Mac's update system is pretty unobtrusive and just not stupid like windows update. Mac wants you to sign up to all of their AI and cloud stuff, but you do not have to. I did not even setup the app store. The only apps that I wanted were Firefox and a system wide EQ for my earbuds. I found eqMac on gethub, the free version works pretty well. Anyway don't let the different OS scare you away, MacOS is pretty decent and not really that much different then windows. I got used to it in 2 days of use. Also it should get 7 years of software support.
Here is a comparison of the Neo with the 4 year old Lenovo Yoga 13 it is replacing. The Lenovo was $550 when new so a very similar price category from 4 years ago. Not sure if this will be useful to anyone so I am putting this at the end, but I figure that a lot of people will be in the same situation switching from a budget windows machine from a few years ago, Overall the Neo is better in almost every way, except for the screen not being able to fold around like the yoga and the lack of a backlit keyboard.
The Neo is much faster, it way outperforms the AMD 4650u in my old lenovo when it comes to loading web pages and videos. My old laptop had started really chugging lately when loading YouTube and twitch videos. Not sure it there is something wrong my old laptop it or if web sites have just gotten that much harder to run.
My old lenovo Yoga 13 has an absurd amount of flex in the plastic chassis, when you pick up one corner of the device the other edge sags visibly, the keyboard flexes noticeably when typing, the entire chassis will twist with very little effort. The chassis has also cracked in several places and I have been having to superglue it back together to keep the chassis from literally falling apart. The Neo on the other hand is completely rigid you can lift it anywhere, no flex in the keyboard, you would have to really try to do anything to this thing, it is amazing for a laptop of any price, much less a $600 one. Unless you really do something terrible to it I expect the Neo chassis to last well beond the lifetime of the hardware.
The battery life on the neo is great, I would say it is about twice that of the lenovo, even though I just put a new battery in my lenovo 6 months ago.
The Bluetooth range on the Neo is at least 2 times better, my earbuds would loose connection to the lenovo if I walked 20 feet away to go to the bathroom. I can walk anywhere in the house and stay connected to the Neo, range of about 40+ feet in the house and about 80+ feet outside. Not only the range is better but also Bluetooth connects faster and just works kind of flawlessly, while in windows I constantly had odd Bluetooth issues. This is with just some basic $30 Anker Bluetooth earbuds.
WiFI range and throughput is also much better then my old laptop
The yogo 13 had a good screen, so I do not find the screen of the Neo a huge upgrade, it is noticeably more vibrant and it is higher res, although I found 1980x1080 to be fine at this size. Also the Yoga 13 screen could fold all of the way back and around into any position you want and it was touchscreen, so some improvements and some downsides. Still the color on the Neo screen is very nice.
The speakers in the Neo are many times better then the the Lenovo, my old Pixel phone even easily outdid the Lenovo, it had impressively bad speakers.
The webcam on the Neo is also a big upgrade over the Lenovo, higher res and just better all around.
The Neo has a macbook keyboard, so generally it is a very good laptop keyboard. It is not my favorite type of keyboard, but it works quite well and is fast to type.
The trackpad is great, it is so much better then any windows computer trackpad that I have used, it functions a bit different so it takes a bit of getting used to, but it is just so much better then windows trackpads.
As the Neo uses a cellphone chip it does not really need any cooling, so there are no vents and the chassis never gets hot or even warm. My old lenovo would get uncomfortably hot when charging and I had to be careful not to block the vents when using it on a bed or blanket, but that is not a problem with the Neo. There are no vents to block.
Overall this thing is just impressive for $600 and I just do not see any windows laptop coming close to competing for a thin and light web machine. Sure if you want something that can game or do more intensive work stuff, but I just wanted something to run a web browser and this does that incredibly well while also being a beautifully well made device.
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Reviewed in the United States on March 29, 2026