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Description
Human DNASE-1 (Deoxyribonuclease 1) ELISA KitThe Human (DNASE 1) Deoxyribonuclease 1 ELISA Kit measures Deoxyribonuclease 1 in samples. The plate has been pre coated with Human DNase 1 antibody. DNase 1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human DNase 1 Antibody is added and binds to DNase 1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated DNase 1 antibody. After incubation unbound Streptavidin HRP is washed
The Human (DNASE-1) Deoxyribonuclease 1 ELISA Kit measures Deoxyribonuclease 1 in samples. The plate has been pre-coated with Human DNase-1 antibody. DNase-1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human DNase-1 Antibody is added and binds to DNase-1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated DNase-1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human DNase-1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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